Journal: Oncology Reports

Article Title: Exploring the role of disulfidptosis-related signatures in immune microenvironment, prognosis and therapeutic strategies of cholangiocarcinoma

doi: 10.3892/or.2026.9042

Figure Lengend Snippet: F-actin and nuclei in HuCCT1 and RBE cells transfected with si-NC, si-CD109 or si-EFNB2 and treated with 5 µM BAY-876 or 1 µM TCEP for 24 h. si, small interfering; NC, negative control; TCEP, Tris-(2-carboxyethyl)-phosphine hydrochloride.

Article Snippet: Cells were then treated with the GLUT1 inhibitor BAY-876 (10 μM; cat. no. HY-100017, MedChemExpress) and/or the reducing agent, Tris-(2-carboxyethyl)-phosphine hydrochloride (TCEP; 20 mM; cat. no. A600974; Sangon Biotech Co., Ltd.) for 6 h at 37°C.

Techniques: Transfection, Negative Control

Journal: The World Allergy Organization Journal

Article Title: Increased glucose transporter 1 contributes to epithelial barrier dysfunction in allergic rhinitis ☆

doi: 10.1016/j.waojou.2025.101158

Figure Lengend Snippet: Inhibiting GLUT1 alleviates the suppression of tight junction marker expression in nasal epithelial cells mediated by HDM. (A–B) Cell immunofluorescence staining for GLUT1, ZO-1 and occluding in nasal epithelial cells treated with PBS, HDM and HDM + BAY876 (n = 6); WB showing GLUT1, ZO-1 and occluding protein expression in nasal epithelial cells among the 3 groups (n = 6). HDM, house dust mite; GLUT1, glucose transporter 1; WB, western blotting. ∗P < 0.05, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.

Article Snippet: To investigate the effects of inhibiting GLUT1 on cellular function, cells were pretreated with 50 nM BAY876 (MedChemExpress, Monmouth Junction, USA) for 6 h before exposure to 20 μg/mL HDM, followed by a combined intervention for 48 h.

Techniques: Marker, Expressing, Immunofluorescence, Staining, Western Blot

Journal: The World Allergy Organization Journal

Article Title: Increased glucose transporter 1 contributes to epithelial barrier dysfunction in allergic rhinitis ☆

doi: 10.1016/j.waojou.2025.101158

Figure Lengend Snippet: GLUT1 inhibitor alleviates allergen-mediated nasal mucosal inflammation and barrier function damage in murine AR model. (A) Representative HE and immunofluorescence images of nasal mucosal in PBS, AR and AR + BAY876 groups (n = 5); (B) Relative fluorescence intensity of GLUT1, ZO-1 and occluding among the 3 groups (n = 5); (C) Relative fluorescence intensity of GLUT1, ZO-1 and occluding among the 3 groups (n = 5); (D) IL-4, IL-13, IL-17A and IFN-γ concentrations in nasal lavage fluid (n = 5). AR, allergic rhinitis; GLUT1, glucose transporter 1; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Article Snippet: To investigate the effects of inhibiting GLUT1 on cellular function, cells were pretreated with 50 nM BAY876 (MedChemExpress, Monmouth Junction, USA) for 6 h before exposure to 20 μg/mL HDM, followed by a combined intervention for 48 h.

Techniques: Immunofluorescence, Fluorescence